It has been widely applied in many fields such as biological 35, chemical analysis, material science 7, 8, and so on. Labelfree surfaceenhanced raman spectroscopy biosensor. Discrimination between ricin and sulphur mustard toxicity. As such, we highlight the capability of raman spectroscopy with different laser spot sizes for use as a noninvasive, rapid, and specific method to perform in situ analysis of primary bovine. Some studies depend on how much light an atom absorbs. Pdf raman spectroscopy cellbased biosensors semantic. Detection of effect of chemotherapeutic agents to cancer. Surfaceenhanced raman spectroscopybased, homogeneous. Surfaceenhanced raman spectroscopy sers is a noninvasive and powerful tool for identification of molecular species. Single cell raman spectroscopy for cell sorting and imaging. Raman spectroscopy can be used for developing cellbased biosensors for monitoring the interaction of cells with drugs. Cellbased microarrays for in vitro toxicology annual. First, we describe a characterization technique for the application of raman microspectroscopy to estimatethewater content in drying samples. A single cell raman spectrum usually contains more than raman bands which provide rich and intrinsic information of the cell e.
A raman spectroscopy cellbased biosensor has been proposed for rapid detection of toxic agents, identification of the type of toxin and prediction of the concentration used. Surface enhanced raman spectroscopy and its application to. Jun 14, 2011 several studies have previously highlighted the potential of raman spectroscopy to provide useful information on key biochemical markers within cells. Urine surfaceenhanced raman spectroscopy for noninvasive. A 532 nm laser was focused through a 100x objective na 0. Raman spectroscopy is a powerfultechnique for studying cellular. Gold nanoaggregates for probing singleliving cell based on surfaceenhanced raman spectroscopy. Nanopore structures have displayed attractive prospects in diverse important applications such as nanopore. Hela immortalised human cervical cells and hacat dermal cells were cultured on. Urine surfaceenhanced raman spectroscopy for noninvasive diabetic detection based on a portable raman spectrometer ye zou 1,2, meizhen huang 1,2,5, kehui wang 3, biao song 1, yang wang 3, jie chen 1, xi liu 1, xia li 1, lulu lin 1 and gaozhong huang 4. Infrared ir and raman spectroscopy ir and raman spectroscopy measure the energy difference of vibrational energy levels in molecules, they are energy sensitive methods. In these devices, the sensing element is a whole cell or even group of cells.
Raman spectroscopy cellbased biosensors europe pmc. All these methods require cell isolation, lysis or fixation, which is timeconsuming and requires. Krishnakumar professor and head department of physics periyar university salem 636 011, india 2. Kien voon kong, weng kee leong, zhiyong lam, tianxun gong, douglas goh, weber kam on lau, malini olivo. Surfaceenhanced raman spectroscopy biosensors northwestern. A perkinelmer uv vis spectrophotometer lambda 750 at. Raman spectroscopy cellbased biosensors directory of.
Peptideguided surfaceenhanced raman scattering probes for localized cell composition analysis. In the 28 years since its discovery, surfaceenhanced raman scattering sers has. Recent studies showed that the method has a potential for use in microbiological research, too. Development of a flow cell based raman spectroscopy technique to overcome photodegradation in human blood. Raman spectroscopy is a powerful tool to reach this goal, being nondestructive analytical technique, allowing also measurements of samples in aqueous environment. Optical fluorescence, absorbance, light scattering, refractive index. The in vitro cell culture environment can impact on cell biochemistry and cell cycle. View pdf journal of biological engineering biomed central. The vibrational signals associated with sers are greatly enhanced in the presence of au or ag nanoparticles, which work as optical amplifiers. Surface enhanced raman spectroscopy and its application. Combined raman spectroscopy and digital holographic. Potential for raman spectroscopy to provide cancer screening.
Using raman spectroscopy to detect malignant changes in tissues accurate, rapid and noninvasive detection and diagnosis of malignant disease in tissues is an important goal of biomedical research. In surfaceenhanced raman scattering sers, the e field is. Raman spectroscopy is a powerful technique for studying cellular biochemistry. Cell basedbiosensors rely on detecting changes in cell behaviour, metabolism, or induction of celldeath following exposure of live cells to toxic agents. Raman spectra were acquired with a 10 s integration time in the spectral range of 400 4000 cm. Cellbased biosensors rely on detecting changes in cell behaviour, metabolism, or induction of cell death following exposure of live cells to toxic. Ramanspectroscopybased biosensing for applications in. Optical methods, such as diffuse reflectance, fluorescence spectroscopy, and raman spectroscopy, have all been investigated as ways to attain this goal. Cell based biosensors rely on detecting changes in cell behaviour, metabolism, or induction of cell death following exposure of live cells to toxic agents. They are based on interactions of electromagnetic radiation and material but the main differences between these two spectroscopic techniques are the physical effects.
Most coarsely, biosensors and other methods of detecting the genetic, immunological or cell based markers associated with cancer can be separated by their transduction mechanism. A raman microspectroscopy study of water and trehalose in. Shellisolated nanoparticle enhanced raman spectroscopy shiners is an advanced sers technique, which uses au or ag amplifiers coated with 2 to 3nmthick silica shells. Keywords optical biosensors surface enhanced raman scattering sers cancer opto. Surfaceenhanced raman scattering sers has become a mature spectroscopic technique with the fast development in the past four decades, and its detection applications in the fields of chemistry, materials science, biochemistry and life sciences are rapidly expanding. Real time monitoring of multiple parameters in mammalian cell. This paper explores the possibility of using raman spectroscopy to discriminate between cancer and noncancer patients through a peripheral blood sample. Monitoring glycosylation metabolism in brain and breast. Variable instrument parameters are few, spectral manipulation is minimal and a simple interpret. View the article pdf and any associated supplements and figures for a period of 48 hours. University of nottingham, school of physics and astronomy, university park.
A hybrid technique between raman spectroscopy and microscopy is known as raman microscopy and this technique is used to records raman maps, using point mapping method, to represent spatial. The strength of raman spectroscopy for invitro analyses is the ability to establish the statistical effects of the drug exposure in real time on human cells of a specific phenotype that is relevant to the efficacy of the drug. Raman spectroscopy is a laserbased scattering technique that permits the analysis of cellular chemical composition dna, rna, lipids, and proteins, molecular markers, and the biological functions of individual cells within a spectral region of 6002000 cm. Methods and applications of raman microspectroscopy to single. Raman spectroscopy has been used to monitor the cell death that was an indication of the presence of the toxic agents 10. Cellbased biosensors based on inteinmediated protein engineering for detection of. Raman spectroscopy is a powerfultechnique for studying cellular biochemistry.
Raman spectroscopy and raman microscopy thermo fisher. Cellbased biosensors rely on detecting changes in cell behaviour, metabolism, or induction of cell death following exposure of live cells to toxic agents. The manifestation of such substrateinduced changes in cell cycle in the raman microspectroscopic profiles of cell cultures is investigated at the level of nucleolus, nucleus and cytoplasm. The availability of a variety of spectroscopes has. Pimporn reokrungruang, itthi chatnuntawech, tararaj dharakul, suwussa bamrungsap. The raman excitation was provided by a diode laser at 532 nm laser quantum, opus, max power 2 w. Samek, almarashi, h telle, laser physics letters volume.
One of the main challenges faced by biodetection systems is the ability to detect and identify a large range of toxins at low concentrations and in short times. Moreover, raman based cardiac endoscopy methods can be utilized for diagnosis and treatment purposes table1. However, research on the development of a sensor that balances signal enhancement, reproducibility, and uniformity has not yet been proposed for practical applications. Raman spectroscopy is an optical method used in multiple scientific fields. Surfaceenhanced raman spectroscopy sers is an approach focusing on the strong enhancement of the raman signal, which further can minimize the influence of autofluorescence 16. Raman spectroscopy of primary bovine aortic endothelial cells. Basic principles and applications basic principles resonance raman scattering surface enhanced raman scattering sers instrumentation spectrometer excitation sources raman in catalysis in situ cells in situ raman of working catalysts c. The optical setup enabling simultaneous raman and holographic imaging requires the use of two different laser sources 8, 36. They are applied to profile a given cell population with respect to the expression of. S1 supporting information a labelfree surfaceenhanced raman spectroscopy biosensor for onsite breast cancer detection using human tears soogeun kima,1, tae gi kimb,1, soo hyun leec,1, wansun kima, ayoung banga, sang woong moonb, jeongyoon songd, jaeho shinb, jae su yuc, samjin choia a. Thus, the characteristics, performance, and analytical capabilities of a raman microscope play an important role in the successful application of the raman microspectroscopy in biosensing research. Additionally, raman spectroscopy can be easily combined with complementary optical approaches, such as holographic microscopy 7, 8, 36. Single cell raman spectroscopy scrs is a noninvasive and labelfree technology, allowing in vivo and multiple parameter analysis of individual living cells.
We report a paperbased surfaceenhanced raman spectroscopy sers biosensor integrating the enrichment capability, namely enpsers biosensor, for the sensitive, labelfree detection of free bilirubin in blood serum for the accurate diagnosis of jaundice and its related diseases. Biosensors 2018, 8, 107 3 of 19 wavenumbers, it is possible to study the mechanism of cardiac hypertrophy. Actinomycin d intercation with lung cancer cells using raman micro spectroscopy. Jan 17, 2019 new raman methods raman imaging, near field scanning optical microscopy snom, tip enhanced raman spectroscopy ters, surface enhanced raman spectroscopy sers have been widely used in. Raman spectroscopy is a powerful technique for studying cellular.
The objective of this research is to improve the performance of raman biosensors using sers techniques andor hcpcf, by applying partial. Materials, applications, and the future the first observations of the raman spectra of pyridine on roughened silver were made in 19741. Raman spectroscopy biosensors can also provide additional information from the timedependent changes of cellular biochemistry. After confirmation of the ability of cv assay for monitoring of cell viability, hepg2 cells treated with anticancer drugs to confirm the results of raman spectroscopy, as well as its potential as a cell based biosensor for screening the effect of drugs on cell viability. Metaloxide surfaceenhanced raman biosensor template. The goal of this work was to determine if raman spectroscopy could be applied for in. Proposed sers structures have thus far been restricted to noble metals such as au and ag. Sep 25, 2018 the in vitro cell culture environment can impact on cell biochemistry and cell cycle. However, on the one hand, the fabrication techniques to obtain sub. Feb 12, 2019 recently, the synergy between raman techniques and biodetection systems resulted in a combined cell based biosensor raman microscopy platform 10,11. Raman spectroscopy as a modern tool for lactose determination. Raman scattering has long been used to analyze chemical compositions in biological systems. Pdf raman spectroscopy cellbased biosensors researchgate.
Raman spectroscopy and imaging for cancer diagnosis. May 03, 20 cell based biosensors rely on the detection and identification of single cells as well as monitoring of changes induced by interaction with drugs andor toxic agents. Raman spectroscopy is a rapid, nondestructive analytical tool to obtain structural information of molecular and composition of matter 1, 2. However, metaloxidebased sers represents a new frontier in the field of biosensing. Surfaceenhanced raman spectroscopy detection of proteinligand binding using dglucose and glucose.
Cell microarrays cmas make use of similar construction principles. Raman spectroscopy on the other hand is an optical method. Recently, another class of sophisticated biosensors, the socalled cell based biosensors is being developed and they hold high promise for future applications. Raman spectroscopy for grading of live osteosarcoma cells. The recent advances in raman microscopy and imaging. Ultrahigh sensitive raman spectroscopy for subnanoscience. Sensors free fulltext raman spectroscopy cellbased biosensors. Thus, the characteristics, performance, and analytical capabilities of a raman microscope play an important role in the successful application of the raman microspectroscopy in biosensing. In order to overcome the weak signal of spontaneous raman scattering, coherent raman. These data support recent reports that raman spectroscopy can be used to distinguish between live and dead cells, as previously reported using raman micro. However, research on the development of a sensor that balances signal enhancement, reproducibility, and uniformity has not yet been proposed for practical. An in vitro study of the interaction of the chemotherapeutic drug actinomycin d with lung cancer cell lines using raman micro spectroscopy. Raman spectroscopy cellbased biosensors pubmed central pmc. Raman spectroscopy to examine aqueous solutions, samples inside glass containers and samples without any preparation, have led to a rapid growth in the application of the technique.
In addition, micro raman measurements do not require preliminary. One of the main challenges faced by biodetection systems is the ability to detectand identify a large range of toxins at low concentrations and in short times. Cellbasedbiosensors rely on detecting changes in cell behaviour, metabolism, or induction of celldeath following exposure of live cells to toxic agents. Research paper confocal raman spectral imaging study of dapt. Rapid identification of staphylococci by raman spectroscopy. Discrimination of dental pulp stem cell regenerative. Ramanspectroscopy biosensors can also provide additional information from the timedependentchanges of. Dnarna and protein microarrays have proven their outstanding bioanalytical performance throughout the past decades, given the unprecedented level of parallelization by which molecular recognition assays can be performed and analyzed. Raman spectroscopy in colorectal cancer diagnostics. The methods described here have also been applied to a second mammalian cell culture fed. Raman spectroscopy sers, which results in up to 108 enhancement. Owing to its high chemical specificity and noninvasive detection capability, raman scattering has been widely employed in cancer screening, diagnosis, and intraoperative surgical guidance in the past ten years. Osteosarcoma is the most common primary malignant bone tumor, and the grading of osteosarcoma cells relies on traditional histopathology and molecular biology methods, which require rna extraction, protein isolation and immunohistological staining. Different toxic chemicals have differenteffects on living cells and induce different timedependent biochemical changes related tocell death mechanisms.
Raman spectroscopy can be used for developing cell based biosensors for monitoring the interaction of cells with drugs. Request pdf ramanspectroscopybased biosensing for applications in ophthalmology cellbased biosensors rely on the detection and identification of single cells as well as monitoring of. Recently, the synergy between raman techniques and biodetection systems resulted in a combined cellbased biosensor raman microscopy platform 10,11. Raman spectroscopy cellbased biosensors europe pmc article. With the thermo scientific dxr3 family of raman instruments, you can use raman spectroscopy, microscopy, and imaging that quickly creates research grade images giving viewers instant information on the chemical, structural and elemental characteristics of their sample. Cellbased biosensors rely on the detection and identification of single cells as well as monitoring of changes induced by interaction with drugs andor toxic agents. Most disease states start with small changes in cellular processes. Review raman spectroscopy cellbased biosensors citeseerx. Peptideguided surfaceenhanced raman scattering probes for.
In this study, we used confocal raman microspectroscopy to measure water content in desiccated cells. Pdf raman spectroscopy cellbased biosensors semantic scholar. They are applied to profile a given cell population with respect to the. Surfaceenhanced raman scattering sers is an ultrasensitive molecular screening technique with greatly enhanced raman scattering signals from trace amounts of analytes near plasmonic nanostructures. Hela immortalised human cervical cells and hacat dermal cells were cultured on three different substrates, conventional. The setup used for the sperm cell analysis is schematized in figure 2. Cellbased biosensors and their application in biomedicine. Pixis application notes teledyne princeton instruments. Surfaceenhanced raman scattering of whole human blood, blood.
Raman spectroscopy cellbased biosensors directory of open. Raman spectroscopy is a multivariate phenomenon that requires statistical analysis to identify the relationship between recorded spectra and the property of interest. Fundamentals and applications of surfaceenhanced raman. The potential advantage of raman spectroscopy cellbased systems is that they are not engineered to respond specifically to a single toxic agent but are free to react to many biologically active compounds. Sep 17, 2009 raman spectroscopy interrogates native biochemistry through the interaction of light with matter, producing a high definition biochemical fingerprint of the target material.
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